The landscape of open source malware analysis tools improves everyday. A malware analysis lab can be thought of as a set of entry points into a tool chain. The main entry points are a file, a URL, a network traffic capture, and a memory image. This talk is an examination of the major open source tools that satisfy the analysis requirements for each of these entry points. Each tool’s output can potentially feed into another tool for further analysis. The linking of one tool to the next in a tool chain allows one to build a comprehensive automated malware analysis lab using open source software. For file analysis, the three major versions of Cuckoo Sandbox will be examined. To analyze a potentially malicious URL, the low-interaction honeyclient, Thug, will be covered. Next, if one has a network capture (PCAP) to analyze, the Bro Network Security Monitor is a great option, and will be covered. Finally, if the analysis target is a memory image, the Volatility Framework will be examined. Each of the inputs and outputs of the tools will be reviewed to expose ways that they can be chained together for the purpose of automation.